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5'...G C T C T T C (N)1↓...3' 3'...C G A G A A G (N)4↑...5'

5'...↓G A T C ...3' 3'... C T A G↑...5

5'...G↓G N C C...3' 3'...C C N G↑G...5'

5'...C C T G C A↓G G...3' 3'...G G↑A C G T C C...5'

5'...A G T↓A C T...3' 3'...T C A↑T G A...5'

5'...C C↓N G G...3' 3'...G G N↑C C...5' The FastDigest ScrFI (Bme1390I) restriction enzyme recognizes CC^NGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: BmrFI, BssKI, BstSCI, MspR9I, StyD4I) .

5'…A↓C C W G G T...3' 3'…T G G W C C↑A...5'

5'…G C A T C (N)5↓...3' 3'…C G T A G (N)9↑...5' The FastDigest SfaNI (BmsI) restriction enzyme recognizes GCATC(5/9)^ sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer .

5'...C↓T R Y A G...3' 3'...G A Y R T↑C...5'

5'...G G C C N N N N↓N G G C C...3' 3'...C C G G N↑N N N N C C G G...5'

5'...C C C↓G G G...3' 3'...G G G↑C C C...5'

5'...C C C↓G G G...3' 3'...G G G↑C C C...5'

5'...T A C↓G T A...3' 3'...A T G↑C A T...5'

5'...A↓C T A G T...3' 3'...T G A T C↑A...5'

5'...A↓C T A G T...3' 3'...T G A T C↑A...5'

5'...G C A T G↓C...3' 3'...C↑G T A C G...5'

5'...A A T↓A T T...3' 3'...T T A↑T A A...5'

5'...C↓C W W G G...3' 3'...G G W W C↑C...5'

5'...A T T T↓A A A T...3' 3'...T A A A↑T T T A...5'

5'...A C N↓G T...3' 3'...T G↑N C A...5'