5'...G↓C T A G C...3' 3'...C G A T C↑G...5' The FastDigest NheI restriction enzyme recognizes G^CTAGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AsuNHI, BmtI)
5'...↓G T S A C ...3' 3'... C A S T G↑...5' The FastDigest NmuCI restriction enzyme recognizes ^GTSAC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Tsp45I)