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FastDigest buffer for 100% activity G buffer for 100% activity LO certified O buffer for 100% activity Recombinant enzyme R buffer for 100% activity Tango buffer for 100% activity Thermal inactivation at 80°C in 10 min DNA polymerase with a high functional similarity to Bst DNA Polymerase and with a strong strand displacement activity.

5'...T T↓C G A A...3' 3'...A A G C↑T T...5'

5'...G↓G G C C C...3' 3'...C C C G G↑G...5'

5'...T↓G T A C A...3' 3'...A C A T G↑T...5'

5'...T↓G T A C A...3' 3'...A C A T G↑T...5'

. 5'...↓G A T C ...3' 3'... C T A G↑...5' Available as a FastDigest enzyme for rapid DNA digestion The Bsp143I (Sau3AI) restriction enzyme recognizes ^GATC sites and cuts best at 37°C in its own unique buffer (Isoschizomers: BfuCI, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, NdeII, Sau3AIm)

. 5'...↓G A T C ...3' 3'... C T A G↑...5' Available as a FastDigest enzyme for rapid DNA digestion The Bsp143I (Sau3AI) restriction enzyme recognizes ^GATC sites and cuts best at 37°C in its own unique buffer (Isoschizomers: BfuCI, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, NdeII, Sau3AIm)

5'...T C G↓C G A...3' 3'...A G C↑G C T...5'

5'...G G N↓N C C...3' 3'...C C N↑N G G...5'

5'...G G N↓N C C...3' 3'...C C N↑N G G...5'

5'...G C T A G↓C...3' 3'...C↑G A T C G...5'

5'...G G A T C (N)4↓...3' 3'...C C T A G (N)5↑...5'

5'...G G A T C (N)4↓...3' 3'...C C T A G (N)5↑...5'

5'...C↓T T A A G...3' 3'...G A A T T↑C...5'

5'...G T A↓T A C...3' 3'...C A T↑A T G...5'

5'...C C A N N N N N↓N T G G...3' 3'...G G T N↑N N N N N A C C...5'

5'...C C A N N N N N↓N T G G...3' 3'...G G T N↑N N N N N A C C...5'

5'...A T↓C G A T...3' 3'...T A G C↑T A...5'

5'...A T↓C G A T...3' 3'...T A G C↑T A...5'

5'...A T↓C G A T...3' 3'...T A G C↑T A...5'