B buffer for 100% activity Blue/white certified FastDigest buffer for 100% activity G buffer for 100% activity High concentration available LO certified O buffer for 100% activity Recombinant enzyme R buffer for 100% activity Tango buffer for 100% activity Thermal inactivation at 65°C in 10 min T4 DNA Ligase catalyzes the formation of phosphodiester bonds between 5‘-P and 3‘-OH of dsDNA fragments.