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5'...A↓C A T G T...3' 3'...T G T A C↑A...5'

5'...A↓C A T G T...3' 3'...T G T A C↑A...5'

5'...A A↓C G T T...3' 3'...T T G C↑A A...5'

5'...A A↓C G T T...3' 3'...T T G C↑A A...5'

. 5'...R G↓G W C C Y...3' 3'...Y C C W G↑G R...5' Available as a FastDigest enzyme for rapid DNA digestion The Psp5II (PpuMI) restriction enzyme recognizes RG^GWCCY sites and cuts best at 37°C in G buffer (Isoschizomers: PpuMI, PspPPI)

5'...C T G C A↓G...3' 3'...G↑A C G T C...5'

5'...C T G C A↓G...3' 3'...G↑A C G T C...5'

5'...C T G C A↓G...3' 3'...G↑A C G T C...5'

5'...R↓G A T C Y...3' 3'...Y C T A G↑R...5'

5'...G A C N↓N N G T C...3' 3'...C T G N N↑N C A G...5'

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